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myNGS Guides® 应用于NGS的CRISPR Pools

强大的CRISPR/Cas系统使靶向测序成为可能


比如基于探针的杂交捕获技术(e.g., myBaits®), CRISPR/Cas-driven靶向测序,在解决对于短分子序列捕获来说过于复杂的基因组区域特别有用。这几种被报道的技术是通用的,并已兼容于短读Illumina、长读PacBio和Oxford Nanopore平台。


由于我们高效的核酸合成技术,Arbor是唯一可提供独特设备,价格合理、专家设计的多达数千个独特序列的gRNA库,非常适合CRISPR/Cas-driven靶向测序。与我们所有的基因组学和合成生物学产品一样,我们提供免费的专家设计咨询,帮助您实现目标。


如果您有意继续CRISPR/Cas-driven目标测序项目,请随时联系我们,定制由Arbor的myNGS Guides®提供的gRNA文库。

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  • Hardigan, A.A. et al. (2018). CRISPR/Cas9-targeted removal of unwanted sequences from small-RNA sequencing libraries. bioRxiv.

  • Nachmanson, D. et a. (2018). Targeted genome fragmentation with CRISPR/Cas9 enables fast and efficient enrichment of small genomic regions and ultra-accurate sequencing with low DNA input (CRISPR-DS). Genome Research.

  • Gabrieli, T. et al. (2018). Selective nanopore sequencing of human BRCA1 by Cas9-assisted targeting of chromosome segments (CATCH). Nucleic Acid Research.

  • Slesarev, A. et al. (2018). CRISPR/Cas9 Targeted Capture Of Mammalian Genomic Regions For Characterization By NGS. bioRxiv.

  • Montefiori, L. et al. (2017). Reducing mitochondrial reads in ATAC-seq using CRISPR/Cas9. Scientific Reports.

  • Bennett-Baker, P.E. & Mueller, JL. (2017). CRISPR-mediated isolation of specific megabase segments of genomic DNA. Nucleic Acid Research.

  • Shin, GW. et al. (2017). CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis. Nature Communications.

  • Tsai, YC. et al. (2017). Amplification-free, CRISPR-Cas9 Targeted Enrichment and SMRT Sequencing of Repeat-Expansion Disease Causative Genomic Regions. bioRxiv.

  • Fujita, T. et al. (2016). Efficient sequence‐specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. Genes to Cells

  • Gu, W. et al. (2016). Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications. Genome Biology.

  • Wu, J. et al. (2016). The landscape of accessible chromatin in mammalian preimplantation embryos. Nature.

  • Jiang, W. et al. (2015). Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters. Nature Communications.

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