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myTags® Labeled Libraries

myTags 打破了传统FISH探针的限制,是具有高度特异性、无重复序列的探针。我们的探针设计专利,可以使用任何已知序列来生成复杂的oligo文库,消除BAC探针的限制和重复序列的影响。定制文库包含数千个独特序列,可以很容易地放大信号并降低背景噪音,使目标区域可视化。myTags® 可以用作immortal文库,客户可以在自己实验室进行探针标记和扩增;myTags® 同样可以为客户提供即用型标记探针。


产品特点

免费探针设计服务 — 为目标区域的探针定制提供免费生物信息设计服务

高效 — 增加短探针对细胞与细胞核的渗透率

特异性 — 消除FISH实验中由非特异性结合与交叉杂交引起的背景信号

一致性 — 设计的探针拥有相近的Tm值,可用于单杂交

标记型探针 — 提供即用型标记探针,标签类型可选

通用性 — 标记有不同标签的探针,可与其他探针配对使用


应用


myTags ® Labeled Libraries


为您的个性化实验项目定制标签探针。可以从几种染料标签中选择一种或多种荧光基团来增强您的探针信号。标记型探针运用于FISH检测分析,为即用型探针,常用批次产量为700 pmol。


产品特点

使用便捷 – 交付的探针可直接用于您的FISH实验

完全定制 – 从多种标签中选择适用标签,标签类型包括荧光基团和半抗原

增加荧光信号强度 – 使用3色荧光基团标记探针,提高信号强度


mytags_label_options.jpg


Cat.No.DescriptionSize Labels
402007myTags 20K single-labeled ssDNA, 1-20K oligos700 pmol1
402010myTags 20K single-labeled ssDNA, 1-20K oligos1,000 pmol1
402035myTags 20K triple-labeled ssDNA, 1-20K oligos500 pmol3
404007myTags 40K single-labeled ssDNA, 20-40K oligos700 pmol1
406007myTags 60K single-labeled ssDNA, 40-60K oligos700 pmol1

FAQs

引用文献

相关产品


What recommendations do you make for successful FISH assays?

Generally we recommend probe densities between 3-10 probes per kilobase for target regions larger than 50kb. For target regions between 10-50kb, probe densities should be on the higher end of that range, and we may recommend using multiple fluorophores per probe to boost the signal.


Do you provide design assistance for FISH probes?

We offer complementary FISH probe design service using our proprietary design algorithm for most types of FISH projects. Please contact us with a brief description of your project, including the name of your study species, genomic coordinates, and any additional information.


Can you design probes for unassembled genomes?

We can work with any sequence to design probes. Please contact us with a brief description of your project, including the name of your study species, genomic coordinates, and any additional information.


How many FISH assays can you get from 500pmol of labeled probes?

The number of assays per library depends on a number of factors including the probe density of your library, the size of your target region, the number of probes in the library, and the FISH protocol. Generally we recommend starting with 10pmol of labeled probes per standard FISH slide and then modifying the input amount based on the initial results.


Which dyes do you recommend?

Atto-550 is our most popular dye labeling option, followed by Atto-488, Atto-594, and Atto-647N. Biotin, Digoxigenin and 6FAM are also popular options that work well in multi-color FISH assays.


Do you offer other labeling options than what is listed on the website?

We may be able to accommodate other labeling options, please contact us for availability.


What FISH protocol should I use with labeled myTags libraries?

myTags FISH libraries are compatible with most FISH protocols. Please contact us for recommendations.


How quickly can you ship my order?

We generally ask for up to 3-4 weeks after an order is placed to ship myTags libraries

  • Beck, S. et al. (2018). Implications of CpG islands on chromosomal architectures and modes of global gene regulation. Nucleic Acids Research.

  • Braz, G.T. et al. (2018) Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution. Genetics.

  • Hou, L. et al. (2018). Chromosome painting and its applications in cultivated and wild rice. BMC Plant Biology.

  • Walczak, M. et al. (2018). ATG8 Is Essential Specifically for an Autophagy-Independent Function in Apicoplast Biogenesis in Blood-Stage Malaria Parasites. mBio.

  • Beagrie et al, (2017). Complex multi-enhancer contacts captured by genome architecture mapping. Nature.

  • Nozawa et al, (2017). SAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs. Cell.

  • Giorgetti et al, (2016). Structural organization of the inactive X chromosome in the mouse. Nature.

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