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myTags ® Immortal Libraries

myTags 打破了传统FISH探针的限制,是具有高度特异性、无重复序列的探针。我们的探针设计专利,可以使用任何已知序列来生成复杂的oligo文库,消除BAC探针的限制和重复序列的影响。定制文库包含数千个独特序列,可以很容易地放大信号并降低背景噪音,使目标区域可视化。myTags® 可以用作immortal文库,客户可以在自己实验室进行探针标记和扩增;myTags® 同样可以为客户提供即用型标记探针。


产品特点

免费探针设计服务 — 为目标区域的探针定制提供免费生物信息设计服务

高效 — 增加短探针对细胞与细胞核的渗透率

特异性 — 消除FISH实验中由非特异性结合与交叉杂交引起的背景信号

一致性 — 设计的探针拥有相近的Tm值,可用于单杂交

标记型探针 — 提供即用型标记探针,标签类型可选

通用性 — 标记有不同标签的探针,可与其他探针配对使用


应用


myTags ® Immortal Libraries


使用myTags提供的标签标记方案,在需要的时候生成标记探针。myTags immortal文库易于扩增,可以提供海量的探针文库。


产品特点

通用性 – 一个标记引物可与所有immortal文库结合

灵活 – 有利于使用新的染料和标签

高产量 – 当客户自己进行标记探针时,可提高探针产量

技术支持 – 对客户的问题进行快速的回应和专业的解答


货号产品名称规格
402001myTags 20K Immortal dsDNA, 1-20K oligos  200 ng
404001myTags 40K Immortal dsDNA, 20-40K oligos200 ng
406001myTags 60K Immortal dsDNA, 40-60K oligos200 ng
408001myTags 80K Immortal dsDNA, 60-80K oligos 200 ng

实验流程

FAQs

引用文献

相关产品


Immortal workflow-小.jpg


Do you have recommended protocols for labeling immortal libraries?

Yes, we provide the myTags labeling protocol with all myTags immortal libraries for performing the labeling process in your own lab.


What recommendations do you make for successful FISH assays?

Generally we recommend probe densities between 3-10 probes per kilobase for target regions larger than 50kb. For target regions between 10-50kb, probe densities should be on the higher end of that range, and we may recommend using multiple fluorophores per probe to boost the signal.


Do you provide design assistance for FISH probes?

We offer complementary FISH probe design service using our proprietary design algorithm for most types of FISH projects. Please contact us for design assistance.


Can I design my own FISH probe libraries to label using the myTags labeling protocol?

We can often accommodate customer-designed probes into the myTags labeling framework. Please contact us for recommendations on the design parameters and other information before designing your probe sequences.


Can you provide Oligopaints libraries?

Yes, we can synthesize immortal probe libraries that can be labeled using the Oligopaints labeling method. Please note these probe libraries are not compatible with the myTags labeling protocol due to sequence requirements of the Oligopaints method.


Can you design probes for unassembled genomes?

We can work with any sequenced data to design probes. Please contact us with a brief description of your project, including the name of your study species, genomic coordinates, and any additional information.


How quickly can you ship my order?

We generally ask for up to 2-3 weeks after an order is placed to ship myTags libraries.

  • Beck, S. et al. (2018). Implications of CpG islands on chromosomal architectures and modes of global gene regulation. Nucleic Acids Research.

  • Braz, G.T. et al. (2018) Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution. Genetics.

  • Hou, L. et al. (2018). Chromosome painting and its applications in cultivated and wild rice. BMC Plant Biology.

  • Li et al., (2016) Divergence between C. melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern. Cytogenetic and Genome Research.

  • Li et al., (2016) Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns. BMC Evolutionary Biology.

  • Han et al., (2015) Chromosome-specific painting in Cucumis species used bulked oligonucleotides. Genetics.

  • B. J. Beliveau, et al., (2012) Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes. Proc Natl Acad Sci U S A.

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