Gel-free small RNA library prep with  reduced bias The NEXTFLEX® Small RNA-Seq Kit v4 uses patent-pending  technology to provide a completely gel-free small RNA  library preparation solution for Illumina® sequencing  platforms. Our approach to reducing bias involves  optimization of the ligation reaction and the use of  dimer reduction oligonucleotides. These changes results  in decreased bias in comparison to NEXTFLEX® Small  RNA-Seq Kit v3 and standard protocols. NEXTFLEX® Small RNA-Seq Kit v4 allows preparation of  libraries ready for sequencing with a simplified workflow  in approximately 5 hours, with as little input as 1 ng of  total RNA. Libraries prepared with this kit have a higher  proportion of mapping reads and unique miRNAs than  leading competitors (Figure 1 and 2)

Small RNA-seq library prep optimized for  automation

By eliminating all gel-based steps even for low input samples,  the NEXTFLEX® Small RNA-Seq Kit V4 is optimized for easy  migration onto automated liquid handling platforms. Currently  methods are available for the Revvity Sciclone® G3 NGSx,  Sciclone G3 NGSx iQ™, and the Zephyr® G3 NGS workstation.

Illumina® small RNA-seq multiplexing with  UDI barcodes 

Up to 384 UDI barcoded primers are included in each kit  for multiplexing. These UDIs have been redesigned allowing  libraries prepared with this kit can be run on any Illumina®  sequencing platform, including NextSeq® and Novaseq®  sequencers using standard Illumina® sequencing primers.

NOVA-5132-XX-MN_v2310_Manual_NEXTFLEX Small RNA-Seq Kit V4 with UDIs.pdf